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O SciELO Data é um repositório multidisciplinar para depósito, preservação e disseminação de dados de pesquisa de artigos submetidos e aprovados para publicação, ou já publicados em periódicos da Rede SciELO, ou depositados no SciELO Preprints. Para mais informação consulte scielo.org.

Antes de realizar qualquer depósito, consulte os Termos e condições de uso do repositório SciELO Data.

SciELO Data es un repositorio multidisciplinario para depositar, preservar y difundir datos de investigación de artículos enviados, aprobados para publicación o ya publicados en revistas de la Red SciELO o depositados en SciELO Preprints. Para obtener más información, consulte scielo.org.

Antes de realizar cualquier depósito, consulte el Términos y condiciones de uso de SciELO Data.

SciELO Data is a multidisciplinary repository for deposition, preservation and dissemination of research data from articles submitted and approved for publication, already published in SciELO Network journals or deposited in SciELO Preprints. For more information visit scielo.org.

Before any deposit, consult the SciELO Data Terms and Conditions of Use.

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Survey+Question.docx
Sep 30, 2024 - Replication Data for: 1229. Determination of knowledge levels and attitudes of clinician veterinarians working in Thrace region towards antibiotic use
MS Word - 36.9 KB - MD5: 1384c571c556c90e3d160ee4bffb0e1c
Survey+Response.tab
Sep 30, 2024 - Replication Data for: 1229. Determination of knowledge levels and attitudes of clinician veterinarians working in Thrace region towards antibiotic use
Tabular Data - 55.7 KB - 59 Variables, 111 Observations - UNF:6:LIgtbOFbz9LkeZK/dejvPg==
1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
Sep 30, 2024 - Journal of Applied Oral Science
Cazotti Pereira, Bruno; Sacramento, Catharina Marques; Sallum, Enilson Antonio; Monteiro, Mabelle de Freitas; Casarin, Renato Corrêa Viana; Casati, Marcio Zaffalon; Silvério, Karina Gonzales, 2024, "1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential", https://doi.org/10.48331/scielodata.EYNIXB, SciELO Data, V1
Periodontal dental ligament mesenchymal stem cells (PDLMSCs) play a major role in the regeneration of periodontal tissue through the neoformation of root cementum and alveolar bone. These cells are highly heterogeneous, and many present low potential to renovate the hard tissue d...
Figure_1.jpg
Figure_1.jpg
Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
JPEG Image - 54.9 KB - MD5: 40c5d848553a5aabe8d5af740c99e723
(A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was perfo...
Figure_2.jpg
Figure_2.jpg
Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
JPEG Image - 72.0 KB - MD5: 5127f4d23613577fd88222d860b27ea1
Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...
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Figure_3.jpg
Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
JPEG Image - 84.3 KB - MD5: 92d0fe4490bc5cf42378a3f3c0dd681b
Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...
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Figure_4.jpg
Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
JPEG Image - 29.2 KB - MD5: a3e1d7015c7d87af5e5d65b2ebe0003e
BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...
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Figure_5.jpg
Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
JPEG Image - 133.5 KB - MD5: 242b65ac7579931697e0a0ab58cc5209
Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...
Figure_S1.jpg
Figure_S1.jpg
Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
JPEG Image - 95.5 KB - MD5: 33a14ad65b0b588017f6154b2054d041
Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...
Figure_S2.jpg
Figure_S2.jpg
Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential
JPEG Image - 245.2 KB - MD5: 41f02eaac2d5d79e1e062cf12fa76638
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