981 to 990 of 1,434 Results
Soares, Igor Paulino Mendes; Anselmi, Caroline; Pires, Maria Luiza Barucci Araújo; Ribeiro, Rafael Antonio de Oliveira; Leite, Maria Luísa; Soares, Diana Gabriela; Costa, Carlos Alberto de Souza; Hebling, Josimeri, 2023, "Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions", https://doi.org/10.48331/scielodata.96UYNU, SciELO Data, V1, UNF:6:IjHO+b7kj6Myf0Mc0pVuhg== [fileUNF]
Background: Simulating a bacterial-induced pulpitis environment in vitro may contribute to exploring mechanisms and bioactive molecules to counteract these adverse effects. Aim: To investigate the chronic exposure of human dental pulp cells (HDPCs) to lipopolysaccharides (LPS) ai... |
Tabular Data - 3.8 KB - 17 Variables, 26 Observations - UNF:6:LSNwFGn+dwi2ez+SuniLog==
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Tabular Data - 7.5 KB - 23 Variables, 26 Observations - UNF:6:EOXFvVKC/SHxlFnGE/tSBw==
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MD5: ea22cae28745d876524ad54498e02320
A) Schematic representation of the experimental design to access the effects of different concentrations of lipopolysaccharides (LPS) on human dental pulp cells (HDPCs). Cells were tested under two different experimental conditions: #1) LPS concentrations diluted in FBS-free α-ME... |
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MD5: 6966e2b3847eda0c6dab4236c0585fbe
Flow cytometry histograms of the mesenchymal stem cell antigens expressed in the established primary culture of human dental pulp cells (HDPCs). Sections A) and B) show representative negativity of FITC and PE channels of fluorescence controls. Representative histograms and perce... |
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A) Cell viability after seven days (% of control FBS-free medium) for both experimental conditions: #1) LPS concentrations diluted in FBS-free α-MEM (basal medium); or #2) LPS concentrations diluted in α-MEM + 10% FBS (growth medium). Columns are means and error bars are standard... |
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MD5: 14ee549758209488a35b53ea7d23f2b3
A) Cell viability (% of 1-day control) of human dental pulp cells (HDPCs) cultured with the LPS concentrations (0 - control, 0.1, 1, and 10 µg/mL) diluted in α-MEM + 10% FBS assessed weekly (on days 1, 7, 14, and 21). Columns are means and error bars are standard deviations (n=8)... |
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MD5: cbf541822fc13cf8c6f0001b8922f6f3
A) Gene expression modulation (fold change of control) of odontogenic markers (ALPL and DSPP) and cytokines (TNF, IL1B, and IL8) by human dental pulp cells (HDPCs) cultured with LPS concentrations (0 - control, 0.1, 1, and 10 µg/mL) on day 7. Columns are means and error bars are... |
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MD5: 2501b3cad13af8db16588738eb8a9c4c
Immunofluorescence of NF-κB subunit p65 (green fluorescence – FITC) and actin filaments (F-actin, red fluorescence – ActinRed555) expressed in human dental pulp cells (HDPCs) after one and seven days of exposure to 0 (control) or 10 µg/mL LPS. The cell nuclei (blue fluorescence –... |
Tabular Data - 471 B - 5 Variables, 11 Observations - UNF:6:nqrJ20BOWltA0ue6ynMMpw==
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