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JPEG Image - 54.9 KB - MD5: 40c5d848553a5aabe8d5af740c99e723
(A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was perfo...
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Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...
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Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...
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BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...
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Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...
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Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...
JPEG Image - 245.2 KB - MD5: 41f02eaac2d5d79e1e062cf12fa76638
Illustration of the project outline
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