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JPEG Image - 72.0 KB - MD5: 5127f4d23613577fd88222d860b27ea1
Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...
JPEG Image - 84.3 KB - MD5: 92d0fe4490bc5cf42378a3f3c0dd681b
Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...
JPEG Image - 29.2 KB - MD5: a3e1d7015c7d87af5e5d65b2ebe0003e
BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...
JPEG Image - 133.5 KB - MD5: 242b65ac7579931697e0a0ab58cc5209
Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...
JPEG Image - 95.5 KB - MD5: 33a14ad65b0b588017f6154b2054d041
Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...
JPEG Image - 245.2 KB - MD5: 41f02eaac2d5d79e1e062cf12fa76638
Illustration of the project outline
PNG Image - 261.3 KB - MD5: c0a5d402324427c99b3df3758881e2c7
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