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Publication Year: 2024

461 to 470 of 1,630 Results

Survey+Question.docx Sep 30, 2024 - Replication Data for: 1229. Determination of knowledge levels and attitudes of clinician veterinarians working in Thrace region towards antibiotic use MS Word - 36.9 KB - MD5: 1384c571c556c90e3d160ee4bffb0e1c
Survey+Response.tab Sep 30, 2024 - Replication Data for: 1229. Determination of knowledge levels and attitudes of clinician veterinarians working in Thrace region towards antibiotic use Tabular Data - 55.7 KB - 59 Variables, 111 Observations - UNF:6:LIgtbOFbz9LkeZK/dejvPg==
1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential Sep 30, 2024 - Journal of Applied Oral Science Cazotti Pereira, Bruno; Sacramento, Catharina Marques; Sallum, Enilson Antonio; Monteiro, Mabelle de Freitas; Casarin, Renato Corrêa Viana; Casati, Marcio Zaffalon; Silvério, Karina Gonzales, 2024, "1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential", https://doi.org/10.48331/scielodata.EYNIXB, SciELO Data, V1 Periodontal dental ligament mesenchymal stem cells (PDLMSCs) play a major role in the regeneration of periodontal tissue through the neoformation of root cementum and alveolar bone. These cells are highly heterogeneous, and many present low potential to renovate the hard tissue d...
Figure_1.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 54.9 KB - MD5: 40c5d848553a5aabe8d5af740c99e723 (A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was perfo...
Figure_2.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 72.0 KB - MD5: 5127f4d23613577fd88222d860b27ea1 Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...
Figure_3.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 84.3 KB - MD5: 92d0fe4490bc5cf42378a3f3c0dd681b Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...
Figure_4.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 29.2 KB - MD5: a3e1d7015c7d87af5e5d65b2ebe0003e BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...
Figure_5.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 133.5 KB - MD5: 242b65ac7579931697e0a0ab58cc5209 Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...
Figure_S1.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 95.5 KB - MD5: 33a14ad65b0b588017f6154b2054d041 Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...
Figure_S2.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 245.2 KB - MD5: 41f02eaac2d5d79e1e062cf12fa76638 Illustration of the project outline

Survey+Question.docx

Sep 30, 2024 - Replication Data for: 1229. Determination of knowledge levels and attitudes of clinician veterinarians working in Thrace region towards antibiotic use

MS Word - 36.9 KB -

Survey+Response.tab

Sep 30, 2024 - Replication Data for: 1229. Determination of knowledge levels and attitudes of clinician veterinarians working in Thrace region towards antibiotic use

Tabular Data - 55.7 KB - 59 Variables, 111 Observations -

1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

Sep 30, 2024 - Journal of Applied Oral Science

Cazotti Pereira, Bruno; Sacramento, Catharina Marques; Sallum, Enilson Antonio; Monteiro, Mabelle de Freitas; Casarin, Renato Corrêa Viana; Casati, Marcio Zaffalon; Silvério, Karina Gonzales, 2024, "1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential", https://doi.org/10.48331/scielodata.EYNIXB, SciELO Data, V1

Periodontal dental ligament mesenchymal stem cells (PDLMSCs) play a major role in the regeneration of periodontal tissue through the neoformation of root cementum and alveolar bone. These cells are highly heterogeneous, and many present low potential to renovate the hard tissue d...

Figure_1.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 54.9 KB -

(A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was perfo...

Figure_2.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 72.0 KB -

Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...

Figure_3.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 84.3 KB -

Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...

Figure_4.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 29.2 KB -

BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...

Figure_5.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 133.5 KB -

Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...

Figure_S1.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 95.5 KB -

Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...

Figure_S2.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 245.2 KB -

Illustration of the project outline

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