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Publication Year: 2024

371 to 380 of 1,363 Results

Figure_1.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 54.9 KB - MD5: 40c5d848553a5aabe8d5af740c99e723 (A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was perfo...
Figure_2.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 72.0 KB - MD5: 5127f4d23613577fd88222d860b27ea1 Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...
Figure_3.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 84.3 KB - MD5: 92d0fe4490bc5cf42378a3f3c0dd681b Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...
Figure_4.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 29.2 KB - MD5: a3e1d7015c7d87af5e5d65b2ebe0003e BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...
Figure_5.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 133.5 KB - MD5: 242b65ac7579931697e0a0ab58cc5209 Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...
Figure_S1.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 95.5 KB - MD5: 33a14ad65b0b588017f6154b2054d041 Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...
Figure_S2.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 245.2 KB - MD5: 41f02eaac2d5d79e1e062cf12fa76638 Illustration of the project outline
README.txt Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential Plain Text - 5.1 KB - MD5: 47c7d64e041ef540e5f55c901592740d
Table_1.txt Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential Plain Text - 635 B - MD5: 0b57c0c03ec08bcca6518c6ecef6f51c Primer sequences used for real-time quantitative PCR amplifications.
01_README_RBSO.txt Sep 29, 2024 - Banco de Dados dos valores dos Benefícios Ocupacionais Brasileiro Plain Text - 1.4 KB - MD5: a46343c9c6c1dad5a23e7a72ab192975

Figure_1.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 54.9 KB -

(A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was perfo...

Figure_2.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 72.0 KB -

Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...

Figure_3.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 84.3 KB -

Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...

Figure_4.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 29.2 KB -

BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...

Figure_5.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 133.5 KB -

Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...

Figure_S1.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 95.5 KB -

Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...

Figure_S2.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 245.2 KB -

Illustration of the project outline

README.txt

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

Plain Text - 5.1 KB -

Table_1.txt

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

Plain Text - 635 B -

Primer sequences used for real-time quantitative PCR amplifications.

01_README_RBSO.txt

Sep 29, 2024 - Banco de Dados dos valores dos Benefícios Ocupacionais Brasileiro

Plain Text - 1.4 KB -

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