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Tabular Data - 132.6 KB - 88 Variables, 416 Observations - UNF:6:mMiYnwJw8K/IZ/wL45vYIg==
Attached is the Excel spreadsheet with the data for STATA.
Plain Text - 5.5 KB - MD5: a2ed1c04eef93753a04b353c02d1f76b
Instruções e esclarecimentos dos dados.
MS Excel Spreadsheet - 28.2 KB - MD5: 89c2f18d6310f6a5e09d370aec4057fd
MS Excel Spreadsheet - 22.0 KB - MD5: ec22d187f03e00ade73c21297cd9e7c9
Figure 1. Primers sequences used for RT-PCR and RT-qPCR.
JPEG Image - 4.6 MB - MD5: eed36ac30a4f1843c5fa4336ab68af16
Figure 2. Isolation and culture of human gingival fibroblasts (HGFs).(A) The appearance of primary HGFs cultured for 2 days.(B) The appearance of primary HGFs cultured for 4 days.(C) The appearance of primary HGFs cultured for 7 days.(D) The appearance of primary HGFs cultured fo...
JPEG Image - 5.9 MB - MD5: de0869fde45f658ac92b5c4ce00aeb92
Figure 3. Identification of the 2th passage cells from HGFs with immunofluorescent staining.(A-C) The green positive expression of FSP-1 in the cytoplasmof HGFs.(D-F) Keratin is unstained in the cytoplasm.(G-I) The positive staining of vimentin in the cytoplasm of HGFs was red fl...
JPEG Image - 975.3 KB - MD5: 46329664c6f49b2e3d5b260470318045
Figure 4. The content of C5a in the cell supernatant of the different group was measured by ELISA assay. Data represent the mean ± SEM. of three independent experiments. +P < 0.05 vs. the control group. *P < 0.05 vs. the LPS+C5aRA group.
JPEG Image - 936.2 KB - MD5: 5a247c4226aa3dcdf08872097e0104dc
Figure 5. Effects of C5aR antagonists on the expression of C5aR and inflammatory factors of LPS-induced HGFs by RT-qPCR and Western blotting.(A) C5aRA inhibited expression of C5aR, TNF-α, IL-1β, and IL-6 mRNA in LPS-stimulated HGFs. (B) C5aR antagonists inhibited expression of C5...
JPEG Image - 1007.0 KB - MD5: 09d779f58bb300917e8f61d7dab2e6f5
Figure 6. Effects of C5aR antagonists on NF-κB signaling pathway activation and TLR4 expression in LPS-stimulated HGFs.(A) Effects of C5aR antagonists on mRNA levels of p65, p-p65, IκBα, p-IκBα and TLR4 in LPS-stimulated HGFs.(B) Effects of C5aR antagonists on protein levels of p...
JPEG Image - 4.5 MB - MD5: 419a93b3d9988aea661ba0fb63c17492
Figure 7. The effects of C5aR antagonists on p-p65 translocation into the nucleus were used to detect by immunoflfluorescence staining.(A-C) LPS+C5aR antagonists group.(D-F) The control group.(G-I) LPS group. The Blue staining represented the nucleus; The green staining was p-p65...
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