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MS Word - 1.8 MB - MD5: cd683ae12b4b0eacf03cec7cbf5823a2
The revised step-by-step guide available on SciELO demonstrates how to calculate percentage values for questions with multiple response options. This update will assist users in properly analyzing the dataset when dealing with multiple-choice questions.
Tabular Data - 11.5 KB - 74 Variables, 60 Observations - UNF:6:+33+DMuVZt0Ie0xHVW3bFA==
This updated version now includes all variable names and descriptions in English, ensuring that other researchers can understand and utilize the dataset more effectively.
JPEG Image - 54.9 KB - MD5: 40c5d848553a5aabe8d5af740c99e723
(A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was perfo...
JPEG Image - 72.0 KB - MD5: 5127f4d23613577fd88222d860b27ea1
Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three tim...
JPEG Image - 84.3 KB - MD5: 92d0fe4490bc5cf42378a3f3c0dd681b
Osteoblast genes expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment indicated that 1,25(OH)2D3 upregulated the expression of mRNAs for RUNX2 (A), ALP (B), and OCN (C). Bars represent mean ± standard deviation (SD) where intergroup analysis statistical...
JPEG Image - 29.2 KB - MD5: a3e1d7015c7d87af5e5d65b2ebe0003e
BMP-2 protein expression. Quantification of BMP-2 secreted in supernatant of LOP 1, LOP 2 and LOP 3 cells after 3, 7 and 14 days of osteogenic induction associated with 1,25(OH)2D3. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences ar...
JPEG Image - 133.5 KB - MD5: 242b65ac7579931697e0a0ab58cc5209
Effect of 1,25(OH)2D3 on mineral nodule deposition. (A-C) Microscopic of Alizarin Red Staining (AR-S) mineralization of LOP 1, LOP 2, and LOP 3, respectively. Scale bar = 40µm. (D-F) Quantification of AR-S at 28 days. Experiments were performed in triplicate three times, with com...
JPEG Image - 95.5 KB - MD5: 33a14ad65b0b588017f6154b2054d041
Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...
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