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Patntirapong, Somying; Champakerdsap, Chunya; Mathaveechotikul, Pichaya; Vatanasilp, Apichaya, 2024, "Replication Data for: Improvement of osteoblast adhesion, viability, and mineralization via restoring cell cytoskeleton after bisphosphonate discontinuation in vitro", https://doi.org/10.48331/scielodata.G7NCSX, SciELO Data, V1, UNF:6:TzhLckb/KHUlQ3pudtqQjw== [fileUNF]
Introduction: Bisphosphonate is prescribed to treat excessive bone resorption in osteoporotic patients. However, its use is associated with potential adverse effects such as medication-related osteonecrosis of the jaw, prompting the introduction of the drug holiday concept in pat... |
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Cell viability of pre-osteoblasts and osteoblasts after ALN treatment and discontinuation at various time points. MC3T3 cells were incubated with 1, 5, and 10 µM ALN (A1, A5, and A10). Negative control was abbreviated as A0. The “S” was abbreviated for stoppage of ALN. N = 4 |
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Examination of osteoblast nodule formation and viability. Osteoblasts were incubated with 1, 5, and 10 µM ALN (A1, A5, and A10). (A) Micrographs demonstrate nodule formation after treatment and cessation of ALN. (B) Quantification of alizarin red-positive area (mm2). (C) Quantifi... |
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Evaluation of viable cells. (A) Micrographs of pre-osteoblast depicting cell morphology. (B) Number of live cells after 7-day cell culture. (C) Percent of dead cells after 7-day cell culture. Arrowhead indicated round cells. Scale bar = 100 µm. N = 4 |
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Investigation of cell adhesion after treatment and cessation of ALN. (A) Confocal images of nuclei and cell cytoskeleton. (B) Number of DAPI/field. (C) Stress fiber intensity. (D) Stress fiber density. (E) Cell area (µm2). (F) Cell perimeter. (G) Area/perimeter (A/P). (H) Aspect... |
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Gene expressions. Two conditions of treatment and cessation were investigated. Gr 1: Tx1d S1d, Gr 9: Tx4d S3d. Dashed lines are mRNA expressions of A0. N = 3 |
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A schematic diagram demonstrates experimental plan. (A) Time periods of ALN treatment and discontinuation for cell viability. (B) Time periods of ALN treatment and discontinuation for mineralization. (C) Cell adhesion and cell cytoskeleton processing |
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MTT assay demonstrates pre-osteoblast cell viability. The cells without treatment grew gradually from 2 days to 7 days. ALN treatments (1, 5, and 10 µM) affected cell viability |
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This study demonstrated the effects of ALN and ALN discontinuation in a pre-osteoblast/an osteoblast. ALN and ALN discontinuation affected cell cytoskeleton (red color), which led to changes in cell morphology, cell adhesion, cell viability, and bone nodule formation. ALN, alendr... |
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