Journal of Applied Oral Science

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1 to 10 of 599 Results

Figure_1.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 110.3 KB - MD5: 9186330d351f4128c47efd53f99678f4 MTT viability assay for determining the doses of ZOL (A), GGOH (B), and PFA (C) to use in rescue assays. (D) ZOL-induced viability loss rescue assay results where UN indicates untreated (set to 100% viability), ZOL indicates 50 microM ZOL. GG10 and GG30 indicate 10 microM and 30...
Figure_2.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 121.2 KB - MD5: b7281b7dea20731da083ec90015eb7d3 Quantitative real-time RTPCR to determine the relative expression of selected SLC genes in HGFs with 18S rRNA gene normalization. The relative amount of (A) SLC20A1, (B) SLC20A2, (C) SLC34A1, (D) SLC34A2, and (E) SLC34A3 transporter gene expression in HGFs compared to the respect...
Figure_3.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 160.1 KB - MD5: f90be0a594d0b2c8498d11115956d605 MTT viability assay to determine the toxicity of Dynasore in HGFs (A). (B) ZOL-induced viability loss rescue assay where UN indicates untreated (set to 100% viability) and the microM Dynasore concentrations are designated as Dyn 10, Dyn 30, etc. and ZOL is 50 microM in concentrat...
Figure_4.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 136.2 KB - MD5: 38ff2dbcf169524a0ac3ae289a7d7a82 Confocal fluorescence microscopy to determine whether dextran and ZOL colocalize in HGFs. From the same experiment, a representative photomicrograph of an HGF in the absence of Dynasore (A, C, E, and G) and a representative photomicrograph of an HGF in the presence of Dynasore (B...
Figure_5.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 238.1 KB - MD5: 1848dacb3313d0438b270a286ed930bd Confocal fluorescence microscopy of live HGFs exposed to LysoSensor Green DND-189 to measure endosomal acidification. Representative field of HGFs in the absence (A) and presence (B) of 30 microM Dynasore in which the exposure settings were kept constant. 20X objective, scale bar...
Figure_6 .jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 111.2 KB - MD5: a264a0113bfaeea8a79565c42aabc60d Measurement of the perinuclear localization of ZOL and the positive control for macropinocytosis, dextran, in the absence and presence of 30 microM Dynasore. Violin plots display the HGF perinuclear proportion of intracellular TRITC-dextran (A) and AF647-ZOL (D) fluorescence. Hor...
Graphical_Abstract.pdf Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification Adobe PDF - 84.1 KB - MD5: 496fd3da516f74ba23d636c4a2e04675 Text summary and data displaying the major findings.
HGFIMIRescue_DataGraph.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 66.2 KB - MD5: db50edfaef672c4413910622875fe627 This graph displays the "data not shown" in the Dynasore rescue portion of Results. An optimized dose of imipramine (IMI) of 25 microM for one hour prior to 72 hours of incubation in the presence of zoledronate does not rescue the 50 microM zoledronate (ZOL)-treated HGFs. Error b...
HGFIMIRescue_PercentViabilityData.tab Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification Tabular Data - 1.9 KB - 11 Variables, 25 Observations - UNF:6:cShYK+KhoIAqGIBEkEIfQQ== This file contains the data used to make the graph that was "data not shown" in the Dynasore rescue portion of Results. The graph is the file entitled "HGFIMIRescue_DataGraph.jpg" and uploaded separately in the dataset. This graph shows that an optimized dose of imipramine (IMI)...
OsteoblastPFARescue_DataGraph.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 62.9 KB - MD5: d3c779177990d91baf6a82fa93ff3a1e This graph shows that optimized doses of phosphonoformic acid (PFA) from 0.25-0.75 mM for either a one-hour pre-treatment (PRE) prior or continual exposure during 72 hours of incubation in the presence of zoledronate do not rescue the 50 microM zoledronate (ZOL)-treated human cal...

Figure_1.jpg

Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification

JPEG Image - 110.3 KB -

MTT viability assay for determining the doses of ZOL (A), GGOH (B), and PFA (C) to use in rescue assays. (D) ZOL-induced viability loss rescue assay results where UN indicates untreated (set to 100% viability), ZOL indicates 50 microM ZOL. GG10 and GG30 indicate 10 microM and 30...

Figure_2.jpg

JPEG Image - 121.2 KB -

Quantitative real-time RTPCR to determine the relative expression of selected SLC genes in HGFs with 18S rRNA gene normalization. The relative amount of (A) SLC20A1, (B) SLC20A2, (C) SLC34A1, (D) SLC34A2, and (E) SLC34A3 transporter gene expression in HGFs compared to the respect...

Figure_3.jpg

JPEG Image - 160.1 KB -

MTT viability assay to determine the toxicity of Dynasore in HGFs (A). (B) ZOL-induced viability loss rescue assay where UN indicates untreated (set to 100% viability) and the microM Dynasore concentrations are designated as Dyn 10, Dyn 30, etc. and ZOL is 50 microM in concentrat...

Figure_4.jpg

JPEG Image - 136.2 KB -

Confocal fluorescence microscopy to determine whether dextran and ZOL colocalize in HGFs. From the same experiment, a representative photomicrograph of an HGF in the absence of Dynasore (A, C, E, and G) and a representative photomicrograph of an HGF in the presence of Dynasore (B...

Figure_5.jpg

JPEG Image - 238.1 KB -

Confocal fluorescence microscopy of live HGFs exposed to LysoSensor Green DND-189 to measure endosomal acidification. Representative field of HGFs in the absence (A) and presence (B) of 30 microM Dynasore in which the exposure settings were kept constant. 20X objective, scale bar...

Figure_6 .jpg

JPEG Image - 111.2 KB -

Measurement of the perinuclear localization of ZOL and the positive control for macropinocytosis, dextran, in the absence and presence of 30 microM Dynasore. Violin plots display the HGF perinuclear proportion of intracellular TRITC-dextran (A) and AF647-ZOL (D) fluorescence. Hor...

Graphical_Abstract.pdf

Adobe PDF - 84.1 KB -

Text summary and data displaying the major findings.

HGFIMIRescue_DataGraph.jpg

JPEG Image - 66.2 KB -

This graph displays the "data not shown" in the Dynasore rescue portion of Results. An optimized dose of imipramine (IMI) of 25 microM for one hour prior to 72 hours of incubation in the presence of zoledronate does not rescue the 50 microM zoledronate (ZOL)-treated HGFs. Error b...

HGFIMIRescue_PercentViabilityData.tab

Tabular Data - 1.9 KB - 11 Variables, 25 Observations -

This file contains the data used to make the graph that was "data not shown" in the Dynasore rescue portion of Results. The graph is the file entitled "HGFIMIRescue_DataGraph.jpg" and uploaded separately in the dataset. This graph shows that an optimized dose of imipramine (IMI)...

OsteoblastPFARescue_DataGraph.jpg

JPEG Image - 62.9 KB -

This graph shows that optimized doses of phosphonoformic acid (PFA) from 0.25-0.75 mM for either a one-hour pre-treatment (PRE) prior or continual exposure during 72 hours of incubation in the presence of zoledronate do not rescue the 50 microM zoledronate (ZOL)-treated human cal...

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