Journal of Applied Oral Science

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121 to 130 of 754 Results

Figure_S1.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 95.5 KB - MD5: 33a14ad65b0b588017f6154b2054d041 Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...
Figure_S2.jpg Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential JPEG Image - 245.2 KB - MD5: 41f02eaac2d5d79e1e062cf12fa76638 Illustration of the project outline
README.txt Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential Plain Text - 5.1 KB - MD5: 47c7d64e041ef540e5f55c901592740d
Table_1.txt Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential Plain Text - 635 B - MD5: 0b57c0c03ec08bcca6518c6ecef6f51c Primer sequences used for real-time quantitative PCR amplifications.
Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification Aug 30, 2024 Daniel Jones, 2024, "Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification", https://doi.org/10.48331/scielodata.QRF50O, SciELO Data, V1, UNF:6:je6g67yoz3qSMg4h+USlmw== [fileUNF] Objective: For treatment of medication-related osteonecrosis of the jaw, one proposed approach is a topical agent to block entry of these medications in oral soft tissues. We tested the ability of phosphonoformic acid (PFA), an inhibitor of bisphosphonate entry through certain so...
Figure_1.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 110.3 KB - MD5: 9186330d351f4128c47efd53f99678f4 MTT viability assay for determining the doses of ZOL (A), GGOH (B), and PFA (C) to use in rescue assays. (D) ZOL-induced viability loss rescue assay results where UN indicates untreated (set to 100% viability), ZOL indicates 50 microM ZOL. GG10 and GG30 indicate 10 microM and 30...
Figure_2.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 121.2 KB - MD5: b7281b7dea20731da083ec90015eb7d3 Quantitative real-time RTPCR to determine the relative expression of selected SLC genes in HGFs with 18S rRNA gene normalization. The relative amount of (A) SLC20A1, (B) SLC20A2, (C) SLC34A1, (D) SLC34A2, and (E) SLC34A3 transporter gene expression in HGFs compared to the respect...
Figure_3.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 160.1 KB - MD5: f90be0a594d0b2c8498d11115956d605 MTT viability assay to determine the toxicity of Dynasore in HGFs (A). (B) ZOL-induced viability loss rescue assay where UN indicates untreated (set to 100% viability) and the microM Dynasore concentrations are designated as Dyn 10, Dyn 30, etc. and ZOL is 50 microM in concentrat...
Figure_4.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 136.2 KB - MD5: 38ff2dbcf169524a0ac3ae289a7d7a82 Confocal fluorescence microscopy to determine whether dextran and ZOL colocalize in HGFs. From the same experiment, a representative photomicrograph of an HGF in the absence of Dynasore (A, C, E, and G) and a representative photomicrograph of an HGF in the presence of Dynasore (B...
Figure_5.jpg Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification JPEG Image - 238.1 KB - MD5: 1848dacb3313d0438b270a286ed930bd Confocal fluorescence microscopy of live HGFs exposed to LysoSensor Green DND-189 to measure endosomal acidification. Representative field of HGFs in the absence (A) and presence (B) of 30 microM Dynasore in which the exposure settings were kept constant. 20X objective, scale bar...

Figure_S1.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 95.5 KB -

Effect of 1,25(OH)2D3 on metabolic activity. LOP 1 cells were cultured in the presence of 0 to 30nM of 1,25(OH)2D3. Metabolic activity is an indicator for cell viability and was measured with MTT assay at 1, 3, 7, and 10 days. Bars represent mean ± standard deviation (SD) where *...

Figure_S2.jpg

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

JPEG Image - 245.2 KB -

Illustration of the project outline

README.txt

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

Plain Text - 5.1 KB -

Table_1.txt

Sep 30, 2024 - 1,25(OH)2D3 increases osteogenic potential of human periodontal ligament cells with low osteoblast potential

Plain Text - 635 B -

Primer sequences used for real-time quantitative PCR amplifications.

Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification

Aug 30, 2024

Daniel Jones, 2024, "Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification", https://doi.org/10.48331/scielodata.QRF50O, SciELO Data, V1, UNF:6:je6g67yoz3qSMg4h+USlmw== [fileUNF]

Objective: For treatment of medication-related osteonecrosis of the jaw, one proposed approach is a topical agent to block entry of these medications in oral soft tissues. We tested the ability of phosphonoformic acid (PFA), an inhibitor of bisphosphonate entry through certain so...

Figure_1.jpg

Aug 30, 2024 - Replication Data for: The dynamin inhibitor, dynasore, prevents zoledronate-induced viability loss in human gingival fibroblasts by partially blocking zoledronate uptake and inhibiting endosomal acidification

JPEG Image - 110.3 KB -

MTT viability assay for determining the doses of ZOL (A), GGOH (B), and PFA (C) to use in rescue assays. (D) ZOL-induced viability loss rescue assay results where UN indicates untreated (set to 100% viability), ZOL indicates 50 microM ZOL. GG10 and GG30 indicate 10 microM and 30...

Figure_2.jpg

JPEG Image - 121.2 KB -

Quantitative real-time RTPCR to determine the relative expression of selected SLC genes in HGFs with 18S rRNA gene normalization. The relative amount of (A) SLC20A1, (B) SLC20A2, (C) SLC34A1, (D) SLC34A2, and (E) SLC34A3 transporter gene expression in HGFs compared to the respect...

Figure_3.jpg

JPEG Image - 160.1 KB -

MTT viability assay to determine the toxicity of Dynasore in HGFs (A). (B) ZOL-induced viability loss rescue assay where UN indicates untreated (set to 100% viability) and the microM Dynasore concentrations are designated as Dyn 10, Dyn 30, etc. and ZOL is 50 microM in concentrat...

Figure_4.jpg

JPEG Image - 136.2 KB -

Confocal fluorescence microscopy to determine whether dextran and ZOL colocalize in HGFs. From the same experiment, a representative photomicrograph of an HGF in the absence of Dynasore (A, C, E, and G) and a representative photomicrograph of an HGF in the presence of Dynasore (B...

Figure_5.jpg

JPEG Image - 238.1 KB -

Confocal fluorescence microscopy of live HGFs exposed to LysoSensor Green DND-189 to measure endosomal acidification. Representative field of HGFs in the absence (A) and presence (B) of 30 microM Dynasore in which the exposure settings were kept constant. 20X objective, scale bar...

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