Journal of Applied Oral Science

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461 to 470 of 754 Results

Suppmentary_table.xls Feb 2, 2023 - A novel PAX9 compound heterozygous variant in a Chinese family with non-syndromic oligodontia and genotype-phenotype analysis of PAX9 variants MS Excel Spreadsheet - 92.0 KB - MD5: 22b8c2fe80952edb3035d4688427be40 PAX9 mutation genotypes and phenotypes
C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways Jan 2, 2023 Chen, Yan; Liu, Yang; Li, Hao; Huna, Risu; Tan, Xiaohan; Li, Ning; Zhang, Yiying; Jiao, Xiaohui; Liu, Mingyue, 2023, "C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways", https://doi.org/10.48331/scielodata.XR5TEV, SciELO Data, V1 Objective: Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been full...
Figure_1.xls Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways MS Excel Spreadsheet - 22.0 KB - MD5: ec22d187f03e00ade73c21297cd9e7c9 Figure 1. Primers sequences used for RT-PCR and RT-qPCR.
Figure_2.jpg Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways JPEG Image - 4.6 MB - MD5: eed36ac30a4f1843c5fa4336ab68af16 Figure 2. Isolation and culture of human gingival fibroblasts (HGFs).(A) The appearance of primary HGFs cultured for 2 days.(B) The appearance of primary HGFs cultured for 4 days.(C) The appearance of primary HGFs cultured for 7 days.(D) The appearance of primary HGFs cultured fo...
Figure_3.jpg Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways JPEG Image - 5.9 MB - MD5: de0869fde45f658ac92b5c4ce00aeb92 Figure 3. Identification of the 2th passage cells from HGFs with immunofluorescent staining.(A-C) The green positive expression of FSP-1 in the cytoplasmof HGFs.(D-F) Keratin is unstained in the cytoplasm.(G-I) The positive staining of vimentin in the cytoplasm of HGFs was red fl...
Figure_4.jpg Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways JPEG Image - 975.3 KB - MD5: 46329664c6f49b2e3d5b260470318045 Figure 4. The content of C5a in the cell supernatant of the different group was measured by ELISA assay. Data represent the mean ± SEM. of three independent experiments. +P < 0.05 vs. the control group. *P < 0.05 vs. the LPS+C5aRA group.
Figure_5.jpg Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways JPEG Image - 936.2 KB - MD5: 5a247c4226aa3dcdf08872097e0104dc Figure 5. Effects of C5aR antagonists on the expression of C5aR and inflammatory factors of LPS-induced HGFs by RT-qPCR and Western blotting.(A) C5aRA inhibited expression of C5aR, TNF-α, IL-1β, and IL-6 mRNA in LPS-stimulated HGFs. (B) C5aR antagonists inhibited expression of C5...
Figure_6.jpg Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways JPEG Image - 1007.0 KB - MD5: 09d779f58bb300917e8f61d7dab2e6f5 Figure 6. Effects of C5aR antagonists on NF-κB signaling pathway activation and TLR4 expression in LPS-stimulated HGFs.(A) Effects of C5aR antagonists on mRNA levels of p65, p-p65, IκBα, p-IκBα and TLR4 in LPS-stimulated HGFs.(B) Effects of C5aR antagonists on protein levels of p...
Figure_7.jpg Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways JPEG Image - 4.5 MB - MD5: 419a93b3d9988aea661ba0fb63c17492 Figure 7. The effects of C5aR antagonists on p-p65 translocation into the nucleus were used to detect by immunoflfluorescence staining.(A-C) LPS+C5aR antagonists group.(D-F) The control group.(G-I) LPS group. The Blue staining represented the nucleus; The green staining was p-p65...
Figure_8.jpg Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways JPEG Image - 975.9 KB - MD5: e31d40908889cc5a560522952d4d06e4 Figure 8. Effects of C5aR antagonists on MAPK signal pathway activation in LPS-stimulated HGFs.(A) Effects of C5aR antagonists on mRNA levels of JNK, p-JNK, c-JUN and p-c-JUN in LPS-stimulated HGFs. (B) Effects of C5aR antagonists on protein levels of JNK, p-JNK, c-JUN, and p-c-J...

Suppmentary_table.xls

Feb 2, 2023 - A novel PAX9 compound heterozygous variant in a Chinese family with non-syndromic oligodontia and genotype-phenotype analysis of PAX9 variants

MS Excel Spreadsheet - 92.0 KB -

PAX9 mutation genotypes and phenotypes

C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

Jan 2, 2023

Chen, Yan; Liu, Yang; Li, Hao; Huna, Risu; Tan, Xiaohan; Li, Ning; Zhang, Yiying; Jiao, Xiaohui; Liu, Mingyue, 2023, "C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways", https://doi.org/10.48331/scielodata.XR5TEV, SciELO Data, V1

Objective: Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been full...

Figure_1.xls

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

MS Excel Spreadsheet - 22.0 KB -

Figure 1. Primers sequences used for RT-PCR and RT-qPCR.

Figure_2.jpg

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

JPEG Image - 4.6 MB -

Figure 2. Isolation and culture of human gingival fibroblasts (HGFs).(A) The appearance of primary HGFs cultured for 2 days.(B) The appearance of primary HGFs cultured for 4 days.(C) The appearance of primary HGFs cultured for 7 days.(D) The appearance of primary HGFs cultured fo...

Figure_3.jpg

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

JPEG Image - 5.9 MB -

Figure 3. Identification of the 2th passage cells from HGFs with immunofluorescent staining.(A-C) The green positive expression of FSP-1 in the cytoplasmof HGFs.(D-F) Keratin is unstained in the cytoplasm.(G-I) The positive staining of vimentin in the cytoplasm of HGFs was red fl...

Figure_4.jpg

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

JPEG Image - 975.3 KB -

Figure 4. The content of C5a in the cell supernatant of the different group was measured by ELISA assay. Data represent the mean ± SEM. of three independent experiments. +P < 0.05 vs. the control group. *P < 0.05 vs. the LPS+C5aRA group.

Figure_5.jpg

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

JPEG Image - 936.2 KB -

Figure 5. Effects of C5aR antagonists on the expression of C5aR and inflammatory factors of LPS-induced HGFs by RT-qPCR and Western blotting.(A) C5aRA inhibited expression of C5aR, TNF-α, IL-1β, and IL-6 mRNA in LPS-stimulated HGFs. (B) C5aR antagonists inhibited expression of C5...

Figure_6.jpg

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

JPEG Image - 1007.0 KB -

Figure 6. Effects of C5aR antagonists on NF-κB signaling pathway activation and TLR4 expression in LPS-stimulated HGFs.(A) Effects of C5aR antagonists on mRNA levels of p65, p-p65, IκBα, p-IκBα and TLR4 in LPS-stimulated HGFs.(B) Effects of C5aR antagonists on protein levels of p...

Figure_7.jpg

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

JPEG Image - 4.5 MB -

Figure 7. The effects of C5aR antagonists on p-p65 translocation into the nucleus were used to detect by immunoflfluorescence staining.(A-C) LPS+C5aR antagonists group.(D-F) The control group.(G-I) LPS group. The Blue staining represented the nucleus; The green staining was p-p65...

Figure_8.jpg

Jan 2, 2023 - C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways

JPEG Image - 975.9 KB -

Figure 8. Effects of C5aR antagonists on MAPK signal pathway activation in LPS-stimulated HGFs.(A) Effects of C5aR antagonists on mRNA levels of JNK, p-JNK, c-JUN and p-c-JUN in LPS-stimulated HGFs. (B) Effects of C5aR antagonists on protein levels of JNK, p-JNK, c-JUN, and p-c-J...

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