Persistent Identifier
|
doi:10.48331/scielodata.KYQGTA |
Publication Date
|
2025-01-28 |
Title
| Replication Data for: Bmal1 knockout aggravates Porphyromonas gingivalis-induced periodontitis by activating the NF-κB pathway |
Author
| Ye Tian (Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Department of Geriatric Stomatology, Chengdu, China) - ORCID: 0009-0003-0913-2261
Xinran Liu (Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Chengdu, China) - ORCID: 0000-0001-8559-591X
Qiuyu Lu (Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Chengdu, China) - ORCID: 0009-0004-4238-3094
Jiaxin Li (Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Chengdu, China) - ORCID: 0000-0002-4373-9695
Tianqi Wang (Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Chengdu, China) - ORCID: 0009-0001-4565-9525
Mei Tian (Sichuan University, West China Healthcare Hospital of Sichuan University, Chengdu, China) - ORCID: 0009-0009-2000-7406
Yan Ding (Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Chengdu, China) - ORCID: 0009-0000-0699-4745
Jinle Li (Sichuan University, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Department of General Clinic, Chengdu, China) - ORCID: 0000-0001-5305-6639 |
Point of Contact
|
Use email button above to contact.
Jinle Li (Department of General Dentistry, State Key Laboratory for Oral Diseases, West China Hospital of Stomatology Sichuan University, Chengdu, Sichuan, China) |
Description
| Circadian rhythm disorders and NF-κB are closely linked and can exacerbate periodontitis; however, the mechanisms through which circadian rhythm-related genes influence periodontitis are not yet fully understood. Objective: We investigated the effect of brain and muscle Arnt-like protein-1 (BMAL1) on the NF-κB pathway and downstream inflammatory factors on periodontitis. In this study, Bmal1 homozygous knockout and periodontitis mouse models were established. Methodology: Bone marrow-derived macrophages (BMDMs) from Bmal1-/- mice were cultured and stimulated with lipopolysaccharides. Bone resorption was detected using micro-computed tomography and histological analyses. Gene and cytokine expression was assessed using quantitative reverse-transcription PCR and ELISA. The nuclear translocation of p65 was detected using immunofluorescence. Results: Our findings indicate that Bmal1 knockout exacerbates periodontitis severity in mice by activating the NF-κB signaling pathway with increased nuclear translocation of p65 (p<0.05), as well as increased expression of Il-1b, Il-6, and Tnfα (p<0.01), along with decreased Nr1d1 expression (p<0.05) in BMDMs under inflammation. Conclusion: These results highlight the protective role of Bmal1 in periodontitis and suggest its potential link to the circadian clock's influence on the disease. |
Subject
| Medicine, Health and Life Sciences |
Keyword
| Circadian Clocks (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D057906
Periodontitis (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D010518
ARNTL Transcription Factors (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D056930
NF-kappa B (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D016328 |
Related Publication
| Tian Y, Liu X, Lu Q, Li J, Wang T, Tian M, Ding Y, Li J. Bmal1 knockout aggravates Porphyromonas gingivalis-induced periodontitis by activating the NF-κB pathway. J Appl Oral Sci. 2025;33:e20240388. doi: 10.1590/1678-7757-2024-0388 https://doi.org/10.1590/1678-7757-2024-0388 |
Depositor
| Amadei, Roberto |
Deposit Date
| 2025-01-21 |