Persistent Identifier
|
doi:10.48331/scielodata.37EU59 |
Publication Date
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2023-03-28 |
Title
| Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse |
Author
| Wang, Si (College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China) - ORCID: 0000-0003-4155-0810
Xiao, Xiaoyue (College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China)
Chen, Jianwei (State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University)
Zhai, Qiming (College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China)
Xin, Liangjing (College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China)
Zheng, Xinhui (College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China)
Song, Jinlin (College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China) - ORCID: 0000-0002-0224-6640 |
Point of Contact
|
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Wang, Si (College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China) |
Description
| Objectives: The mid-palatal expansion technique is commonly used to correct maxilla constriction in dental clinics. There is, however, a tendency for it to relapse, and the key molecules responsible for modulating bone formation remain elusive. Thus, this study aimed to investigate whether signal transducer and activator of transcription 3 (STAT3) activation contributes to osteoblast-mediated bone formation during palatal expansion and relapse. Methodology: Thirty male Wistar rats were randomly allocated into Ctrl (control), E (expansion only), and E+Stattic (expansion plus STAT3-inhibitor, Stattic) groups. Micro-computed tomography, micromorphology staining, and immunohistochemistry of the mid-palatal suture were performed on days 7 and 14. In vitro cyclic tensile stress (10% magnitude, 0.5 Hz frequency, and 24 h duration) was applied to rat primary osteoblasts and Stattic was administered for STAT3 inhibition. The role of STAT3 in mechanical loading-induced osteoblasts was confirmed by alkaline phosphatase (ALP), alizarin red staining, and western blots. Results: The E group manifested a greater arch width than the E+Stattic group after expansion, and the differences between the two groups remained significant after relapse. Active bone formation was detected in the E group with increased expression of ALP, COL-I, and Runx2, whereas the expression of osteogenesis-related factors was downregulated in the E+stattic group. After STAT3 inhibition, expansive force-induced bone resorption was attenuated, as demonstrated by TRAP staining. Furthermore, the administration of Stattic in vitro partially suppressed tensile stress-enhanced osteogenic markers in osteoblasts. Conclusions: Overall, STAT3 inactivation reduced osteoblast-mediated bone formation during palatal expansion and post-expansion relapse, suggesting that it may be a potential therapeutic target to treat force-induced bone formation. (2023-03-25) |
Subject
| Medicine, Health and Life Sciences |
Keyword
| Osteogenesis (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D010012
Palatal Expansion Technique (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D010155
STAT3 Transcription Factor (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D050796 |
Related Publication
| Xiao X, Chen, J, Zhai Q, Xin L, Zheng X, Wang J, et al. Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse. J App Oral Sci. 2023;31:e20230009. doi: 10.1590/1678-7757-2023-0009 doi: 10.1590/1678-7757-2023-0009 https://dx.doi.org/10.1590/1678-7757-2023-0009 |
Depositor
| Wang, Si |
Deposit Date
| 2023-03-25 |