Replication Data for to "Titanium micro-nano textured surface with strontium incorporation improves osseointegration. An in vivo and in vitro study".

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Replication Data for to "Titanium micro-nano textured surface with strontium incorporation improves osseointegration. An in vivo and in vitro study".
Costa Filho, Pio Moerbeck da; Marcantonio, Camila Chiérici; Oliveira, Diego Pedreira de; Lopes, Maria Eduarda Scordamaia; Puetate, Julio Cesar Sanchez; Faria, Luan Viana; Carvalho, Letícia de Freitas; Molon, Rafael Scaf de; Garcia Junior, Idelmo Rangel; Nogueira, Andressa Vilas Bôas; Deschner, James; Cirelli, Joni Augusto, 2024, "Replication Data for to "Titanium micro-nano textured surface with strontium incorporation improves osseointegration. An in vivo and in vitro study".", https://doi.org/10.48331/scielodata.LCXGHX, SciELO Data, V2, UNF:6:aZz6As6qZ0gpKp4oZ+RCXA== [fileUNF]
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Description
This study aimed to investigate the osseointegration of titanium (Ti) implants with micro-nano textured surfaces functionalized with strontium additions (Sr) in a pre-clinical rat tibia model. Ti commercially pure (cp-Ti) implants were installed bilaterally on the tibia of 64 Holtzman rats, divided into four experimental groups (n=16/group): (1) Machined surface - control (C); (2) Micro-nano textured surface treatment (MN); (3) Micro-nano textured surface with Sr2+ addition (MNSr); (4) Micro-nano textured surface with a higher complementary addition of Sr2+ (MNSr+). Two experimental euthanasia periods were assessed at 15 and 45 days (n=8/period). The tibia was subjected to micro-computed tomography (μ-CT), histomorphometry with the EXAKT system, removal torque (TR) testing, and gene expression analysis by PCR-Array of 84 osteogenic markers. Gene expression and protein production of bone markers were performed in an in vitro model with MC3T3-E1 cells. The surface characteristics of the implants were evaluated by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and laser scanning confocal microscopy. SEM, confocal, and EDS analyses demonstrated the formation of uniform micro-nano textured surfaces in the MN group and Sr addition in the MNSr and MNSr+ groups. TR test indicated greater osseointegration in the later period for treated surfaces. Histological analysis highlighted the benefits of the treatments, especially in cortical bone, where there was an increase in bone-implant contact in groups MN (15 days) and MNSr (45 days) compared to the control group. Gene expression analysis of osteogenic activity markers showed modulation of various osteogenesis-related genes. According to the in vitro model, RT-qPCR and ELISA demonstrated that the treatments favored gene expression and production of osteoblastic differentiation markers. In conclusion, micro-nano textured surface and Sr addition can effectively improve and accelerate implant osseointegration and is, therefore, an attractive approach to modifying titanium implant surfaces with significant potential in clinical practice. (2024-07-31)
Subject Medicine, Health and Life Sciences
Keyword Osseointegration, Titanium, Strontium, Dental Implants
Related Publication Costa PM Filho, Marcantonio CC, Oliveira DP, Lopes ME, Puetate JC, Faria LV, et al. Titanium micro-nano textured surface with strontium incorporation improves osseointegration: an in vivo and in vitro study. J Appl Oral Sci. 2024;32:e20240144. doi: 10.1590/1678-7757-2024-0144
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Figure 1- Schematic drawing of the implant with the area represented by the “cortical bone” space measured by two valleys (in red) and the “cancellous bone” space measured by three valleys (in green).
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Figure 2- SEM micro images at 10.000 × magnifications of the surface technological groups after treatment on the titanium implants of groups C (a); MN (b), MNSr (c), and MNSr+ (d). Below is Sr mapping by EDS (i; j). e–h show laser scanning confocal microscopy 3D reconstruction images of surface groups. Moreover, e–h shows a representative area of 128 µm × 128 µm, identifying peaks and valleys with at least 200 nm of resolution in height. It is possible to verify the moderately rougher groups MN, MNSr, and MNSr+ compared to the machined conditions
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Figure 3- Means and standard deviations (SD) of removal torque analysis in N/cm of implants with different surface treatments at 15 and 45 days.
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Figure 4- Means and standard deviations of (a) bone volume fraction (BV/TV), (b) Trabecular bone thickness (Tb.Th), (c) Trabecular Separation (Tb.Sp), and (d) Trabecular Number (Tb.N) in the cortical and medullary bone region of implants with different surface treatments at 15 and 45 days.
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Figure 5- Histological slides for all examined surfaces. (a–d) cortical thread region and (f–g) medullary thread region. (a, e) Control; (b, f) MN surface; (c, g) MNSr surface; (d, h) MNSr+ at 15 days. Magnification at 10×.
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Figure 6- Histometric analysis comprising BIC and BAFO measurements in the regions corresponding to the cortical and medullary bones. Distinct superscript letters (upper case compares the periods in the column, lower case compares the surface treatments in the row) indicate statistical difference by the two-way ANOVA followed by the Tukey’s test (p<0.05). In the graphical representation, * indicates a significant difference between the periods in the same group (p<0.05) and # a significant difference between groups (p<0.05).
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Figure 7- Heat Maps demonstrating the comparison and fold changes of the genes diagram of genes associated with osteogenesis of groups. Fold change comparisons between MN (a), MNSr (b), and MNSr+ (c) groups in contrast to the control group. The layout of the PCR array can be observed in (d).
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Figure 8- Scatter Plot generated using Qiagen’s PCR Array Data Analysis Web Portal, which serves to contrast the normalized expression levels of individual genes on the PCR Array between the treated experimental groups in relation to the control group. In this representation, genes exhibiting upregulation are situated in the upper left quadrant denoted by yellow dots, whereas genes displaying downregulation are located in the lower right quadrant represented by blue dots. The black dots represent genes whose expression remains unchanged. Moreover, the accompanying Volcano Plots on the right-hand side depict statistical significance, measured in terms of P-values, juxtaposed with the extent of change, expressed as fold change, observed in the analyzed genes. These comparisons specifically pertain to the fold change values between MN (a), MNSr (b), and MNSr+ (c) groups in contrast to the control group. Detailed values of fold changes, accompanied by their respective statistical significance, are presented in panel (d).
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Figure 9- A. Fold change graphs of genes related to the osteogenic pathway evaluated at the 7-day timepoint. B. ELISA test of culture medium collected from cell supernatants after 7 days of cell culture. The concentration was expressed in pg/ml and normalized according to the number of cells (fg/cells). * indicates statistical difference between groups by the Kruskal-Wallis test followed by the Dunn’s multiple comparisons test (p<0.05).
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