Replication Data for: The effect of injectable platelet-rich fibrin and platelet-rich fibrin in regenerative endodontics: a comparative in vitro study

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Replication Data for: The effect of injectable platelet-rich fibrin and platelet-rich fibrin in regenerative endodontics: a comparative in vitro study
Beizhan, Jiang; Jing Pan; Linjuan Luo; Zhen Jiang; Haiyan Huang, 2024, "Replication Data for: The effect of injectable platelet-rich fibrin and platelet-rich fibrin in regenerative endodontics: a comparative in vitro study", https://doi.org/10.48331/scielodata.DTJVO6, SciELO Data, V1, UNF:6:8WHq9xHYoN2E4WbbGnGDRw== [fileUNF]
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Description
Objective: To explore the feasibility of injectable platelet-rich fibrin (i-PRF) in regenerative endodontics by comparing the effect of i-PRF and platelet-rich fibrin (PRF) on biological behaviors and angiogenesis of human stem cells from apical papilla (SCAPs). Methodology: i-PRF and PRF were obtained from venous blood at two different centrifugation ways, followed by hematoxylin-eosin (HE) staining and scanning electron microscope (SEM). Enzyme-linked immunosorbent assay (ELISA) was conducted to quantify the growth factors. SCAPs were cultured with different concentrations of i-PRF extract (i-PRFe) and PRF extract (PRFe), and the optimal concentrations were selected by the Cell Counting Kit-8 (CCK-8) assay. Then the cell proliferation and migration potentials of SCAPs were observed using the CCK-8 assay and the Transwell assay. The mineralization ability was detected by alizarin red staining (ARS) and angiogenesis capacity was detected by tube formation assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to evaluate the genes expression related to mineralization and angiogenesis. Data were subjected to statistical analysis. Results: i-PRF and PRF presented a similar three-dimensional fibrin structure while i-PRF released a higher concentration of growth factors than PRF (P < .05). 1/4× i-PRFe and 1/4× PRFe were selected as the optimal concentrations. The cell proliferation rate of i-PRFe group was higher than that of PRFe group (P < .05), while no statistical difference was observed in cell migration between them (P > .05). More importantly, our results showed that i-PRFe had a stronger effect on SCAPs than PRFe in facilitating mineralization and angiogenesis with the consistent result of RT-qPCR (P < .05). Conclusion: This work revealed that i-PRF released a higher concentration of growth factors and was superior to PRF in promoting proliferation, mineralization and angiogenesis of SCAPs, suggesting that i-PRF could be a promising biological scaffold applied in pulp regeneration. (2024-04-09)
Subject Medicine, Health and Life Sciences
Keyword Platelet-Rich Fibrin, Angiogenesis, Regenerative Endodontics
Related Publication Pan J, Luo L, Jiang Z, Huang H, Jiang B. The effect of injectable platelet-rich fibrin and platelet-rich fibrin in regenerative endodontics: a comparative in vitro study. J Appl Oral Sci. 2024:e20230449. doi: 10.1590/1678-7757-2023-0449
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FIGURE 1
Tabular Data - 540 B
Published May 2, 2024
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3 Variables, 10 Observations UNF:6:21YANWkoC7FJeI/39GgS5g==
Primer sequences used in the RT-qPCR reaction.
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FIGURE 2.jpg
FIGURE 2.jpg
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Published May 2, 2024
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Structures and growth factors of i-PRF and PRF. The microstructures of i-PRF (A) and PRF (B) were observed by hematoxylin-eosin (HE) staining, in which fibrin fibrils were stained pink, while platelets and leukocytes were stained dark blue (scale bar = 100 µm). Leu: leukocytes; RBC: red blood cells. The ultrastructures of i-PRF (C) and PRF (D) were observed by scanning electron microscopy (SEM) (scale bar = 20 µm). (E) The growth factors PDGF-BB, IGF1, TGF-β1 and VEGF were detected by enzyme-linked immunosorbent assay (ELISA). Results are expressed as mean ± standard deviation. (**P<.01, ***P<.001)
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FIGURE 3.jpg
FIGURE 3.jpg
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Published May 2, 2024
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Effect of different concentrations of i-PRFe (A) and PRFe (B) on the proliferation of SCAPs for 1, 3 and 5 days. Results are expressed as mean ± standard deviation of the OD value. (*P<.05, **P<.01, ***P<.001)
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FIGURE 4.jpg
FIGURE 4.jpg
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Published May 2, 2024
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Evaluation of proliferation and migration of SCAPs after culturing with i-PRFe or PRFe. (A) Statistical analysis of the proliferation proportions of different groups after culturing for 1, 3 and 5 days. Results are expressed as mean ± standard deviation of the OD value. (B) The average number of migrating cells from different groups per field was statistically analyzed after 24 h. Results are expressed as mean ± standard deviation of the OD value. (C) The representative images of the crystal violet-stained migratory cells from different groups were captured by light microscopy (scale bar = 500 µm). (*P<.05, ***P<.001)
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Effect of i-PRFe and PRFe on odonto/osteogenic differentiation of SCAPs. (A) Representative images of alizarin red staining (ARS) of SCAPs after culturing with different media for 7, 14 and 21 days. (B) Mineralization quantification was analyzed, and the results are expressed as mean ± standard deviation of the OD value. (C) Relative gene expression levels of ALP, DMP-1, DSPP and OCN after culturing with different media for 7, 14 and 21 days. Results are expressed as mean ± standard deviation. (*P<.05, **P<.01, ***P<.001)
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Effect of i-PRFe and PRFe on angiogenic differentiation of SCAPs. (A) Representative images of immunofluorescence (IF) staining of SCAPs after culturing with different media for 6 h. (B) The quantification of angiogenesis was determined using ImageJ software, and the results were shown with the ratio of tubular area to total area. (C) Relative gene expression levels of ANG, CXCR4, VEGF and PDFGFRA after culturing with different media for 6 h. Results are expressed as mean ± standard deviation. (*P<.05, **P<.01, ***P<.001)
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README
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Table_1.tab
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Published May 2, 2024
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3 Variables, 5 Observations UNF:6:ovWgNVLtAvO0kI50LnnOdQ==
Quantification of growth factors in i-PRF and PRF (Mean ± standard deviation)
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Table_10.tab
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4 Variables, 6 Observations UNF:6:hleSoBgBf1Va7lLnzVWtYg==
Relative OCN expression level (Mean ± standard deviation)
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Table_11.tab
Tabular Data - 107 B
Published May 2, 2024
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2 Variables, 5 Observations UNF:6:lU59Q9R4VUQUa+nxe/mFUQ==
Relative tubular area in angiogenesis (Mean ± standard deviation)
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