Replication Data for: Three-dimensional Measurement of the Depth of Invasion in Oral Squamous Cell Carcinoma Samples using Lugol’s Iodine-enhanced Micro-computed Tomography: An Original Study

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Jiaxin, Yu; Gu, Zhouyu; Wang, Lichan; Zhang, Qian; Pu, Yumei; Hu, Qingang; Xia, Chengwan; Wang, Yuxin, 2024, "Replication Data for: Three-dimensional Measurement of the Depth of Invasion in Oral Squamous Cell Carcinoma Samples using Lugol’s Iodine-enhanced Micro-computed Tomography: An Original Study", https://doi.org/10.48331/scielodata.4NM6WS, SciELO Data, V1

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Description	Objectives: Depth of invasion (DOI) in oral squamous cell carcinoma (OSCC) guides treatment and prognosis but lacks three-dimensional (3D) insight. Thus, this study aimed to investigate the feasibility and accuracy of using Lugol’s iodine-enhanced micro-computed tomography (CT) for the 3D measurement of DOI in OSCC samples. Methods: Fifty in vitro OSCC samples from Nanjing Stomatological Hospital (July 2022 to January 2024) were subjected to micro-CT imaging with a slice thickness of 50 µm following 3% Lugol iodine staining for 12 h, followed by pathological examination and staining. The panoramic diagnostic scanner digitally measured the pathological DOI. The micro-CT DOI was measured by evaluating the voxel value of the boundary of the tumor lesion and comparing it with the pathological examination results. Experienced physicians analyzed both measurements, and statistical analyses were performed to determine their correlation. Lugol iodine-enhanced micro-CT imaging distinguishes various tissue structures, such as tumor tissue, epithelial tissue, muscle tissue, blood vessel structure, and other major tissue structures in 3D space. Results: This imaging technique identified and localized micro-tumor lesions (1.82 × 1.5 × 1 mm3) when used in conjunction with pathological sections. Statistical analysis indicated a strong correlation between pathological DOI and micro-CT DOI (P < .001; r = 0.986). During the measurement of DOI information, Lugol iodine-enhanced micro-CT imaging effectively compensated for the loss of 3D space information in the pathological measurements, thereby improving the accuracy of the DOI measurement. Conclusions: Lugol iodine-enhanced micro-CT improves OSCC DOI 3D measurements, enhances pathological staging accuracy, and aids treatment decisions and prognosis. (2024-11-07)
Subject	Medicine, Health and Life Sciences
Keyword	X-Ray Microtomography, Lugol's solution, Squamous Cell Carcinoma of Head and Neck, Imaging, Three-Dimensional
Related Publication	Yo J, Gu Z, Wang L, Zhang Q, Pu Y, Hu Q, et al. Three-dimensional measurement of the depth of invasion in oral squamous cell carcinoma samples using Lugol’s iodine-enhanced micro-computed tomography: an original study. J Appl Oral Sci. 2025;33:e20240304. doi: 1678-7757-2024-0304
License/Data Use Agreement	CC BY 4.0

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	Figure_1.jpg JPEG Image - 1.5 MB Published Nov 7, 2024 0 Downloads MD5: 262cd1094021de9c5220362aec47680f Lugol's iodine-enhanced micro-CT three-dimensional measurement DOI research flow chart The in vitro OSCC samples were fully soaked in freshly prepared 3% Lugol's iodine for 12 hours. Subsequently, the samples were scanned using micro-CT. After scanning, the samples were digitally scanned following the routine pathological section production process, and the r-DOI and p-DOI were measured respectively.	Preview "Figure_1.jpg" Access File File Access Public Download Options JPEG Image Download Metadata Data File Citation EndNote XML RIS BibTeX
	Figure_2.jpg JPEG Image - 2.6 MB Published Nov 7, 2024 0 Downloads MD5: 0495b31796aa7b560f79c742f3132ad0 Measuring the depth of invasion from the deepest point of invasion reconstructed basement membrane line. The black two-way arrow solid line represents the depth of invasion (DOI).The dashed line represents the tumor boundary.	Preview "Figure_2.jpg" Access File File Access Public Download Options JPEG Image Download Metadata Data File Citation EndNote XML RIS BibTeX
	Figure_3.jpg JPEG Image - 4.7 MB Published Nov 7, 2024 0 Downloads MD5: ea17797f7054adc0dfaaf55d14fae74b Lugol's iodine-enhanced micro-computed tomography and pathological images of oral squamous cell carcinoma in vitro sample. The dashed line represents the tumor boundary. The black boxes correspond to the enlarged parts of the local tissue in the (C) and (D) figures.	Preview "Figure_3.jpg" Access File File Access Public Download Options JPEG Image Download Metadata Data File Citation EndNote XML RIS BibTeX
	Figure_4.jpg JPEG Image - 4.3 MB Published Nov 7, 2024 0 Downloads MD5: 7a2b1b3ba596e654b2c4e464a26e50ee Lugol’s iodine-enhanced micro-computed tomography (CT) can identify minimal residual lesions. The dashed line represents the tumor boundary (A) The tumor is outlined by a dotted line. (B) Magnification of tumor site on micro-CT. (C) Pathological image of the same section. (D) Magnification of tumor site on pathological section	Preview "Figure_4.jpg" Access File File Access Public Download Options JPEG Image Download Metadata Data File Citation EndNote XML RIS BibTeX
	Figure_5.jpg JPEG Image - 3.8 MB Published Nov 7, 2024 0 Downloads MD5: bc8aa14c4d2dcc451278375c3977cf5e Depth of invasion (DOI) could be measured accurately by Lugol’s iodine-enhanced three-dimensional micro-CT imaging. The dashed line represents the tumor boundary. Double arrows with solid black lines indicate DOI. (A) 3D micro-computed tomography (CT) image (the red region was the tumor region). (B) Deepest radiological (r)-DOI measurement on micro-CT. (C) Pathological (p)-DOI measurement on pathological images. (D) r-DOI measurement on the micro-CT image corresponds to pathological images in (C).	Preview "Figure_5.jpg" Access File File Access Public Download Options JPEG Image Download Metadata Data File Citation EndNote XML RIS BibTeX
	Figure_6.jpg JPEG Image - 483.6 KB Published Nov 7, 2024 0 Downloads MD5: 72dc717844b510bdb19f680b8d11d02f Correlation between p-depth of invasion (DOI) and r-DOI	Preview "Figure_6.jpg" Access File File Access Public Download Options JPEG Image Download Metadata Data File Citation EndNote XML RIS BibTeX
	README.txt Plain Text - 4.9 KB Published Nov 7, 2024 0 Downloads MD5: b84b74dc3b67eb3bf955670897b480d9	Preview "README.txt" Access File File Access Public Download Options Plain Text Download Metadata Data File Citation EndNote XML RIS BibTeX
	Table_1.txt Plain Text - 1.2 KB Published Nov 7, 2024 0 Downloads MD5: 7ff9d451fefa9d0b7da1df10ccd4eee3 Patients’ characteristics and the change of T-stage	Preview "Table_1.txt" Access File File Access Public Download Options Plain Text Download Metadata Data File Citation EndNote XML RIS BibTeX
	Table_S1.txt Plain Text - 2.0 KB Published Nov 7, 2024 0 Downloads MD5: f7fde732eccdca7003ac6c75a0ecae98 Characteristics of patients and voxel value of tissues	Preview "Table_S1.txt" Access File File Access Public Download Options Plain Text Download Metadata Data File Citation EndNote XML RIS BibTeX

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Persistent Identifier	doi:10.48331/scielodata.4NM6WS
Publication Date	2024-11-07
Title	Replication Data for: Three-dimensional Measurement of the Depth of Invasion in Oral Squamous Cell Carcinoma Samples using Lugol’s Iodine-enhanced Micro-computed Tomography: An Original Study
Author	Jiaxin, Yu (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China) - ORCID: 0009-0006-9863-2859 Gu, Zhouyu (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China) Wang, Lichan (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China) Zhang, Qian (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China) Pu, Yumei (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Head and Neck Oncology Surgery, Nanjing, China) Hu, Qingang (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China) Xia, Chengwan (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China) - ORCID: 0000-0003-3937-2605 Wang, Yuxin (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China) - ORCID: 0000-0002-1629-4176
Point of Contact	Use email button above to contact. Jiaxin, Yu (Nanjing University, Research Institute of Stomatology, Affiliated Hospital of Medical School, Nanjing Stomatological Hospital, Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery, Nanjing, China)
Description	Objectives: Depth of invasion (DOI) in oral squamous cell carcinoma (OSCC) guides treatment and prognosis but lacks three-dimensional (3D) insight. Thus, this study aimed to investigate the feasibility and accuracy of using Lugol’s iodine-enhanced micro-computed tomography (CT) for the 3D measurement of DOI in OSCC samples. Methods: Fifty in vitro OSCC samples from Nanjing Stomatological Hospital (July 2022 to January 2024) were subjected to micro-CT imaging with a slice thickness of 50 µm following 3% Lugol iodine staining for 12 h, followed by pathological examination and staining. The panoramic diagnostic scanner digitally measured the pathological DOI. The micro-CT DOI was measured by evaluating the voxel value of the boundary of the tumor lesion and comparing it with the pathological examination results. Experienced physicians analyzed both measurements, and statistical analyses were performed to determine their correlation. Lugol iodine-enhanced micro-CT imaging distinguishes various tissue structures, such as tumor tissue, epithelial tissue, muscle tissue, blood vessel structure, and other major tissue structures in 3D space. Results: This imaging technique identified and localized micro-tumor lesions (1.82 × 1.5 × 1 mm3) when used in conjunction with pathological sections. Statistical analysis indicated a strong correlation between pathological DOI and micro-CT DOI (P < .001; r = 0.986). During the measurement of DOI information, Lugol iodine-enhanced micro-CT imaging effectively compensated for the loss of 3D space information in the pathological measurements, thereby improving the accuracy of the DOI measurement. Conclusions: Lugol iodine-enhanced micro-CT improves OSCC DOI 3D measurements, enhances pathological staging accuracy, and aids treatment decisions and prognosis. (2024-11-07)
Subject	Medicine, Health and Life Sciences
Keyword	X-Ray Microtomography (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D055114 Lugol's solution (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=C010389 Squamous Cell Carcinoma of Head and Neck (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D000077195 Imaging, Three-Dimensional (MeSH) https://meshb.nlm.nih.gov/record/ui?ui=D021621
Related Publication	Yo J, Gu Z, Wang L, Zhang Q, Pu Y, Hu Q, et al. Three-dimensional measurement of the depth of invasion in oral squamous cell carcinoma samples using Lugol’s iodine-enhanced micro-computed tomography: an original study. J Appl Oral Sci. 2025;33:e20240304. doi: 1678-7757-2024-0304 https://doi.org/10.1590/1678-7757-2024-0304
Depositor	Jiaxin, Yu
Deposit Date	2024-11-07

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